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KMID : 0615720010180020150
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Jouranl of Korean Acupuncture and Moxibustion society
2001 Volume.18 No. 2 p.150 ~ p.160
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Effect of Carthami - Flos aquacupuncture on t-Butylhydroperoxide - induced inhibition of Na+-K+-ATPase activity in cerebral synaptosomes
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Abstract
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Objectives ; This study was undertaken to determine whether Carthami-Flos aquacapuncture (CFA) exerts protective effect against oxidant-induced inhibition of Na^+-K^+-ATPase activity in cerebral synaptosomes.
Methods and Results ; The enzyme activity was dependent on incubation time and enzyme protein concentrations. An oxidant t-butylhydroperoxide (tBHP) at 1 mM concentration caused a significant inhibition of Na^+-K^+-ATPase activity, which was prevented by addition of 0.01% CFA. tBHP inhibition and CFA protection were independent on incubation time or enzyme protein concentrations. The enzyme activity was increased by ATP in a dose dependent manner. Effects of tBHP and CFA were not affected by ATP cocentrations. tBHP (1 mM) produced a significant increase in lipid peroxidation in cerebral synaptosomes, which was prevented by 0.01% CFA. CFA decreased oxygen free radicals generated induced by the phorbol-ester in a dose-dependent manner in human neutrophil.
Conclusions ; These results suggest that CFA exerts protective effect against tBHP-induced inhibition of Na^+-K^+-ATPase activity, which is due to by an antioxidant action resulting from a direct scavenging effect of oxygen free radicals in the cerebral synaptosomes.
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